Killer Bees bear resemblance to killer bees from the real world, and their characteristic appear to be inspired from them. Killer Bees move around very quickly, allowing them to evade any lock on attacks. They fire large, nearly inevitable rainbow beams that can heavily damage Fox's Arwing. Some Killer Bees leave behind a Shield Ring when defeated.
In Star Fox Adventures, Killer Bees briefly appear during the Arwing missions. Unlike Star Fox 64, they no longer shoot out rainbow beams.